Fig 2: SAA1/TLR2 axis induces Rac GTPase-mediated actin reorganization and migration of HSCs(A) Time course of GTPase-Rac1 activation(s) in LX-2 cells at indicated time interval(B and C) Basal and stimulated levels of GTPase-Rac1 in WT and TLR2-/- cells as determined by pull-down assays(D and E) WT and TLR2-/- LX-2 cells were pretreated with NSC 23766 (50 µ?), and activation of GTP-Rac1 was determined by pull-down assay (D), and MLCK and p-MLC at Ser19 activity was determined by western blotting (E).(F and G) Migration assays showing pretreatment of the cells with NSC 23766 (50 µ?) attenuated their migration(s) in agarose spot (F) and Transwell (G) assays. (H) Time course of PI3K activations after treatment of LX-2 cells at indicated time points.(I and J) WT and TLR2-/-cells were pretreated with LY294002 (10 µ?), and the phosphorylation of PI3K at p85 subunits was determined by western blot analysis, and the activations of Rac GTPase were determined by pull-down assay.(K and L) Migration assays showing pretreatment of the cells with LY294002 attenuated their migration(s) in agarose spot (K) and Transwell (L) assays. Photographs are representatives of (n = 6). (Scale bar represents 200 µm).Data represent mean ± SEM *p < 0.01 and **p < 0.001 (n = 3). Photographs are representatives of (n = 6). (Scale bar represents 200 µm). Data represent mean ± SEM *p < 0.01 and **p < 0.001 (n = 3).